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antibodies 158 against pdpn  (Boster Bio)


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    Boster Bio antibodies 158 against pdpn
    Antibodies 158 Against Pdpn, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies 158 against pdpn/product/Boster Bio
    Average 90 stars, based on 4 article reviews
    antibodies 158 against pdpn - by Bioz Stars, 2026-03
    90/100 stars

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    RT-PCR analysis. Selected mRNA expression in sorted LECs from db/db and control mice hearts. Some mRNA levels significantly increase in db/db mice compared to controls. Statistically significant differences ( p -value < 0.05) are marked (*). Abbreviations: Acta2—Actin 2/smooth muscle actin; Bax—BCL2 Associated X; Bcl2—B-cell CLL/lymphoma 2; CCL21—Chemokine (C-C motif) ligand 21; CEACAM1—Carcinoembryonic antigen-related cell adhesion molecule 1; CPT1A—Carnitine Palmitoyltransferase 1A; CX3CL1—chemokine (C-X3-C motif) ligand 1; eNOS—endothelial nitric oxide synthase;Fbn1—fibrillin-1; FOXO1—Forkhead Box O1; GATA2—GATA-binding factor 2; ICAM-1—intercellular adhesion molecule 1; KLF2—Krüppel-like Factor 2; MMP2—Metalloproteinase 2; <t>PDPN—podoplanin;</t> PTEN—phosphatase and tensin homolog deleted on chromosome ten; Reln—reelin; Sdc4—syndecan 4; Snail1—Snail family transcriptional repressor-1; Snail2—Snail family transcriptional repressor-2; VCAM-1—vascular cell adhesion molecule 1; VE-cadherin—Vascular Endothelial Cadherin.
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    RT-PCR analysis. Selected mRNA expression in sorted LECs from db/db and control mice hearts. Some mRNA levels significantly increase in db/db mice compared to controls. Statistically significant differences ( p -value < 0.05) are marked (*). Abbreviations: Acta2—Actin 2/smooth muscle actin; Bax—BCL2 Associated X; Bcl2—B-cell CLL/lymphoma 2; CCL21—Chemokine (C-C motif) ligand 21; CEACAM1—Carcinoembryonic antigen-related cell adhesion molecule 1; CPT1A—Carnitine Palmitoyltransferase 1A; CX3CL1—chemokine (C-X3-C motif) ligand 1; eNOS—endothelial nitric oxide synthase;Fbn1—fibrillin-1; FOXO1—Forkhead Box O1; GATA2—GATA-binding factor 2; ICAM-1—intercellular adhesion molecule 1; KLF2—Krüppel-like Factor 2; MMP2—Metalloproteinase 2; <t>PDPN—podoplanin;</t> PTEN—phosphatase and tensin homolog deleted on chromosome ten; Reln—reelin; Sdc4—syndecan 4; Snail1—Snail family transcriptional repressor-1; Snail2—Snail family transcriptional repressor-2; VCAM-1—vascular cell adhesion molecule 1; VE-cadherin—Vascular Endothelial Cadherin.
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    Identification of 16 cell clusters with diverse annotations reveals a high abundance of <t>PDPN(+)</t> CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.
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    Identification of 16 cell clusters with diverse annotations reveals a high abundance of <t>PDPN(+)</t> CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.
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    Image Search Results


    RT-PCR analysis. Selected mRNA expression in sorted LECs from db/db and control mice hearts. Some mRNA levels significantly increase in db/db mice compared to controls. Statistically significant differences ( p -value < 0.05) are marked (*). Abbreviations: Acta2—Actin 2/smooth muscle actin; Bax—BCL2 Associated X; Bcl2—B-cell CLL/lymphoma 2; CCL21—Chemokine (C-C motif) ligand 21; CEACAM1—Carcinoembryonic antigen-related cell adhesion molecule 1; CPT1A—Carnitine Palmitoyltransferase 1A; CX3CL1—chemokine (C-X3-C motif) ligand 1; eNOS—endothelial nitric oxide synthase;Fbn1—fibrillin-1; FOXO1—Forkhead Box O1; GATA2—GATA-binding factor 2; ICAM-1—intercellular adhesion molecule 1; KLF2—Krüppel-like Factor 2; MMP2—Metalloproteinase 2; PDPN—podoplanin; PTEN—phosphatase and tensin homolog deleted on chromosome ten; Reln—reelin; Sdc4—syndecan 4; Snail1—Snail family transcriptional repressor-1; Snail2—Snail family transcriptional repressor-2; VCAM-1—vascular cell adhesion molecule 1; VE-cadherin—Vascular Endothelial Cadherin.

    Journal: Pathophysiology

    Article Title: Metabolic Syndrome-Driven Changes in Cardiac Lymphatic Endothelium: mRNA Expression and Emerging Questions

    doi: 10.3390/pathophysiology33010004

    Figure Lengend Snippet: RT-PCR analysis. Selected mRNA expression in sorted LECs from db/db and control mice hearts. Some mRNA levels significantly increase in db/db mice compared to controls. Statistically significant differences ( p -value < 0.05) are marked (*). Abbreviations: Acta2—Actin 2/smooth muscle actin; Bax—BCL2 Associated X; Bcl2—B-cell CLL/lymphoma 2; CCL21—Chemokine (C-C motif) ligand 21; CEACAM1—Carcinoembryonic antigen-related cell adhesion molecule 1; CPT1A—Carnitine Palmitoyltransferase 1A; CX3CL1—chemokine (C-X3-C motif) ligand 1; eNOS—endothelial nitric oxide synthase;Fbn1—fibrillin-1; FOXO1—Forkhead Box O1; GATA2—GATA-binding factor 2; ICAM-1—intercellular adhesion molecule 1; KLF2—Krüppel-like Factor 2; MMP2—Metalloproteinase 2; PDPN—podoplanin; PTEN—phosphatase and tensin homolog deleted on chromosome ten; Reln—reelin; Sdc4—syndecan 4; Snail1—Snail family transcriptional repressor-1; Snail2—Snail family transcriptional repressor-2; VCAM-1—vascular cell adhesion molecule 1; VE-cadherin—Vascular Endothelial Cadherin.

    Article Snippet: To detect mRNA levels of selected genes, the following TaqMan Gene Expression Assays were used: CD36: Mm00432403_m1; Pten: Mm00477208_m1; Foxo1: Mm00490671_m1; Klf2: Mm00500486_g1; Bax: Mm00432051_m1; Bcl2: Mm00477631_m1; Cpt1a: Mm01231183_m1; Ccl21a: Mm03646971_gH; Cx3cl1: Mm00436454_m1; Icam1: Mm00516023_m1; Vcam1: Mm01320970_m1; Emilin1: Mm00467244_m1; Mmp2: Mm00439498_m1; Reln: Mm00465200_m1; Snai1: Mm00441533_g1; Snai2: Mm00441531_m1; Acta2: Mm00725412_s1; Cdh5: Mm00546194_s1; Gata2: Mm00492301_m1; Cldn5: Mm00727012_s1; Ceacam1: Mm04204476_m1; Pdpn: Mm01348912_g1; Fbn1: Mm00435217_m1; Nos3: Mm00435217_m1; Sdc4: Mm00488527_m1.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Binding Assay

    LVs localized subepicardially and perivascularly in left ventricles of hearts from control ( a – h ) and db/db ( i – p ) mice. Most of the lymphatic vessels express all three markers: Lyve-1 (red), Pdpn (white) and CD31 (green) (merge; yellow arrows); few Pdpn-positive cells do not express Lyve-1 or CD31 (merge; white arrows). Merged panels ( d , h , l , p ) include DAPI staining of nuclei (blue). ( m – p ) shows zoomed region of ( i – l ). ( q ) shows relative quantification of mRNA level for Pdpn. ( r ) presents the percentage of Pdpn-positive LVs among all cardiac LVs, as shown in representative confocal microscope images. Scale bars—100 µm. Abbreviations: LV—lymphatic vessel, Pdpn—podoplanin. Statistically significant differences ( p -value < 0.05) are marked (*).

    Journal: Pathophysiology

    Article Title: Metabolic Syndrome-Driven Changes in Cardiac Lymphatic Endothelium: mRNA Expression and Emerging Questions

    doi: 10.3390/pathophysiology33010004

    Figure Lengend Snippet: LVs localized subepicardially and perivascularly in left ventricles of hearts from control ( a – h ) and db/db ( i – p ) mice. Most of the lymphatic vessels express all three markers: Lyve-1 (red), Pdpn (white) and CD31 (green) (merge; yellow arrows); few Pdpn-positive cells do not express Lyve-1 or CD31 (merge; white arrows). Merged panels ( d , h , l , p ) include DAPI staining of nuclei (blue). ( m – p ) shows zoomed region of ( i – l ). ( q ) shows relative quantification of mRNA level for Pdpn. ( r ) presents the percentage of Pdpn-positive LVs among all cardiac LVs, as shown in representative confocal microscope images. Scale bars—100 µm. Abbreviations: LV—lymphatic vessel, Pdpn—podoplanin. Statistically significant differences ( p -value < 0.05) are marked (*).

    Article Snippet: To detect mRNA levels of selected genes, the following TaqMan Gene Expression Assays were used: CD36: Mm00432403_m1; Pten: Mm00477208_m1; Foxo1: Mm00490671_m1; Klf2: Mm00500486_g1; Bax: Mm00432051_m1; Bcl2: Mm00477631_m1; Cpt1a: Mm01231183_m1; Ccl21a: Mm03646971_gH; Cx3cl1: Mm00436454_m1; Icam1: Mm00516023_m1; Vcam1: Mm01320970_m1; Emilin1: Mm00467244_m1; Mmp2: Mm00439498_m1; Reln: Mm00465200_m1; Snai1: Mm00441533_g1; Snai2: Mm00441531_m1; Acta2: Mm00725412_s1; Cdh5: Mm00546194_s1; Gata2: Mm00492301_m1; Cldn5: Mm00727012_s1; Ceacam1: Mm04204476_m1; Pdpn: Mm01348912_g1; Fbn1: Mm00435217_m1; Nos3: Mm00435217_m1; Sdc4: Mm00488527_m1.

    Techniques: Control, Staining, Quantitative Proteomics, Microscopy

    Identification of 16 cell clusters with diverse annotations reveals a high abundance of PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.

    Journal: Journal of Inflammation Research

    Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

    doi: 10.2147/JIR.S549953

    Figure Lengend Snippet: Identification of 16 cell clusters with diverse annotations reveals a high abundance of PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.

    Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

    Techniques: Control, Expressing, Immunofluorescence

    PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium exhibit enhanced migration and invasion capabilities. ( A ) Flow cytometric analysis of PDPN and CD90 double-positive primary FLSs populations in OA (up) and PVNS (down) synovium. ( B ) PDPN and CD90 immunofluorescence image of OA FLSs and PVNS FLSs. Red: CD90, Green: PDPN, Blue: nucleus. ( C ) Transwell migration (lower panel) and invasion (upper panel) assays of OA FLSs and PVNS FLSs. ( D ) Statistical analysis of transwell migration (lower panel) and invasion (upper panel) assays. ( E ) Wound-healing analysis of OA FLSs (up) and PVNS FLSs (down). ****P < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

    doi: 10.2147/JIR.S549953

    Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium exhibit enhanced migration and invasion capabilities. ( A ) Flow cytometric analysis of PDPN and CD90 double-positive primary FLSs populations in OA (up) and PVNS (down) synovium. ( B ) PDPN and CD90 immunofluorescence image of OA FLSs and PVNS FLSs. Red: CD90, Green: PDPN, Blue: nucleus. ( C ) Transwell migration (lower panel) and invasion (upper panel) assays of OA FLSs and PVNS FLSs. ( D ) Statistical analysis of transwell migration (lower panel) and invasion (upper panel) assays. ( E ) Wound-healing analysis of OA FLSs (up) and PVNS FLSs (down). ****P < 0.0001.

    Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

    Techniques: Migration, Immunofluorescence

    PDPN(+) CD90(+) sublining fibroblasts express higher levels of pro-inflammatory cytokines and invasion related proteins. ( A ) Volcano plot of DEGs in PDPN(+) CD90(+) sublining fibroblasts between the OA and PVNS synovium. ( B ) KEGG pathway analysis of the DEGs. ( C ) UMAP plots showing the expression of inflammatory factors in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( D ) UMAP plots showing the expression of osteoclast-related proteins in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( E ) Representative images of hub protein expression detected by Western blot. ( F ) Statistical analysis of Western blot. All data are expressed as mean ± SD, n = 3. ns P > 0.05; ***P < 0.001; ****P < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

    doi: 10.2147/JIR.S549953

    Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts express higher levels of pro-inflammatory cytokines and invasion related proteins. ( A ) Volcano plot of DEGs in PDPN(+) CD90(+) sublining fibroblasts between the OA and PVNS synovium. ( B ) KEGG pathway analysis of the DEGs. ( C ) UMAP plots showing the expression of inflammatory factors in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( D ) UMAP plots showing the expression of osteoclast-related proteins in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( E ) Representative images of hub protein expression detected by Western blot. ( F ) Statistical analysis of Western blot. All data are expressed as mean ± SD, n = 3. ns P > 0.05; ***P < 0.001; ****P < 0.0001.

    Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

    Techniques: Expressing, Western Blot